FastQCFastQC Report
Wed 5 Aug 2020
H5JW7AFX2_n01_ncm9-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JW7AFX2_n01_ncm9-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8924494
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT93550.042.5267871
GCGTGAA167650.042.08669766
TGCGTGA136800.040.1927765
CGTGAAA180300.039.81326767
CTGCGTG159850.039.520564
GGCGTGA40000.038.324165
GCGTCGG42600.036.64233460
CTGCTTG195350.036.2085264
CGTCGGC43750.036.07915561
GTCGGCG43900.035.716762
TCGGCGT57050.035.70468563
GCTTGAA204750.035.1786666
TGCTTGA193150.034.66395665
CGGCGTC104650.034.58112758
GTGAAAA236100.034.1690468
GTCTGCG74900.034.15807762
GCCGTCT210250.034.14188856
GCCGGCT89900.033.7144556
GAAAAAA548800.033.56426270
TCTGCGT193150.033.5223863
Produced by FastQC (version 0.11.5)