FastQCFastQC Report
Fri 19 May 2017
H5JVVBGX2_n02_4t-brugia_2017-05-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JVVBGX2_n02_4t-brugia_2017-05-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117758056
Sequences flagged as poor quality0
Sequence length150
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG11389770.9672179031216345No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA3642730.30934019495022913No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3386900.28761514201627103No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC2677920.22740864540087175No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG2618460.22235930932827222No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC2584290.21945759702419002No Hit
CTCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG2582180.21927841607711324No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG2481470.21072613494910278No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT2440390.20723762627331416No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA2298720.19520702685513083No Hit
CTACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTGACGTGACG2263720.19223483105053976No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT2233050.1896303383269167No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2181280.18523403613252581No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTT2061850.1750920548484598No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA2039490.17319324632872676No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC2038550.17311342164140345No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGTGGGTC2011620.17082652926947098No Hit
CGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGCC1922660.16327205673300177No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1911600.162332842858751No Hit
CGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC1798250.1527071744458825No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA1787540.15179768252967762No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG1762390.14966194754437862No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1743670.14807224738832306No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1689090.14343732033076362No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT1624840.1379812180323357No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1621380.13768739524708187No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC1609210.1366539203058855No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1604930.13629046321892407No Hit
CGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC1588510.1348960787871702No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA1502070.12755560434863156No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC1499960.1273764234015548No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1489090.12645334430452895No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT1468550.12470908996663464No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG1461540.12411380160691511No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG1455980.12364164707338579No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT1448600.12301493835801772No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG1431640.12157469719099304No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1412240.11992725151644826No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG1408900.11964361911681015No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA1396060.11855324785592589No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT1395020.11846493118058946No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT1391290.11814818002770018No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT1361670.11563285317821484No Hit
CCGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGC1335190.11338417475234136No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGT1331160.11304194763541273No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA1273730.10816499891947945No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG1266990.10759263892739535No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1264820.10740836278751069No Hit
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC1257350.10677401128293083No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGA1247050.10589933651757974No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1199770.10188432458497787No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG1197690.10170769123430502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA194900.074.5297241
CCTGGCC522750.066.1396565
CATTCGA2755050.064.062399
GTGCGTC230200.062.8788579
TTACGAC238350.061.016575
CCTCACC902200.059.003161
CCAGACG379200.057.2853666
CTCAGAC222950.057.1177141
GACCCAT3154400.056.2195975
CTCACCC942400.056.0982322
GTTACGA264600.054.8812834
TGGCCCG632100.054.6686677
CAACCAG393300.054.6065523
CGACCCA3346550.053.6272854
CCTCAAC178300.053.2632451
CGCGTCT65650.050.8496861
CAGGCGT245700.050.7065477
ACGACTT289850.050.0061077
GACAGGC250850.049.7470365
TGCAAGT297550.046.2920042
Produced by FastQC (version 0.11.2)