FastQCFastQC Report
Fri 19 May 2017
H5JVVBGX2_n02_2c-brugia_2017-05-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JVVBGX2_n02_2c-brugia_2017-05-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107438819
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2127710.19803922081459216No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1971910.18353794451147123No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1828020.1701452060823565No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC1817900.1692032746562488No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA1591630.14814291657468795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1505910.14016442232113516No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1390290.12940294885408224No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1363410.12690105984876845No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1344700.12515960362520365No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG1322530.12309610365318702No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1169100.10881541801013282No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1167380.10865532689818566No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1137110.10583790948037133No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1076500.10019655930879137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT319750.080.128451
CTTGTTA523700.073.419131
TTACGAC608550.062.9517675
GTTACGA623700.061.490744
ATCAAAG556850.050.1597376
CAAAGGA564650.047.9035728
TCAAAGG659450.046.89497
ACGACTT832700.046.2605677
CCAATCA581100.045.2409673
TGTTACG886150.043.287223
CTCAGAC234050.042.5146751
CAAACGA743500.040.82399
CCTGGCC553600.039.7583545
GACAGGC243950.039.4510275
CAGGCGT243300.039.175437
CAATCAA687600.039.1438264
GACTTTT1016600.037.8537039
TCCACTC886050.037.050832
ACTCCTG936000.035.457235
CCTTGTT239550.035.351321
Produced by FastQC (version 0.11.2)