FastQCFastQC Report
Fri 19 May 2017
H5JVVBGX2_n01_3t-brugia_2017-05-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JVVBGX2_n01_3t-brugia_2017-05-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115446521
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT4132810.35798480233111574No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC2931650.25394009058098854No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2811270.24351275167486425No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC2737340.23710892076167456No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG2176070.1884916046972087No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2049580.1775350164081601No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1906800.16516738516529225No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1697630.14704903926901358No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1687660.14618543593877548No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA1664750.14420096730329363No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1654990.14335555421371252No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA1631430.14131478245238763No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1631000.14127753576913765No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1562310.1353275946704362No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG1549430.13421192657680867No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG1505200.13038071541367624No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT1392300.1206012955557145No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1332180.11539368951620464No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1331360.11532266095744886No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC1313660.11378948353064706No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1310750.1135374187672576No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1290400.11177469782740357No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT1258420.10900458403592776No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT1212230.10500359729333031No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1208340.10466664474020831No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1203720.10426645944575498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA723450.087.352731
GTCCAAT291500.086.139391
GTTACGA824300.077.1564564
TTACGAC833300.076.365315
CTCAGAC365250.068.509851
CCAGACG629400.067.0179446
GACAGGC377550.065.844045
CCTGGCC957250.065.49945
CAGGCGT383200.064.8540657
GTGCGTC359850.064.001119
CAACCAG713350.057.973143
ATCAAAG482150.057.397846
CCTCAAC305900.057.2260671
ACAGGCG453400.054.89246
TGGCCCG1166650.053.711867
ACGACTT1211400.052.494357
CAGACAG472000.051.633523
AGACGTG277450.051.0410734
GACTTTT1285800.049.4236079
ACGTGGC286200.049.379316
Produced by FastQC (version 0.11.2)