FastQCFastQC Report
Fri 19 May 2017
H5JVVBGX2_n01_2c-brugia_2017-05-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JVVBGX2_n01_2c-brugia_2017-05-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107438819
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2296480.21374769579326816No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG2249900.20941220509879208No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1956770.18212877042142467No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC1870290.17407953823468592No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA1605250.1494106147983626No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1500010.13961527257666523No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1492690.13893395458861102No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1478440.13760761834137436No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG1452380.1351820518429191No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1242690.11566489761954662No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1234200.11487468044487718No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1167420.10865904994730072No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1164050.10834538305935773No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1121220.1043589282194176No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1081850.10069451712792934No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT312600.080.946781
CTTGTTA507450.072.640471
GTTACGA574200.064.496694
TTACGAC582650.063.5978785
ATCAAAG517050.053.3984956
CAAAGGA532350.050.024288
TCAAAGG616150.049.226487
CTCAGAC212200.048.7978021
GACAGGC212950.047.9058345
CAGGCGT214750.047.3699347
ACGACTT828000.044.9177367
CCAATCA587450.044.525593
GTGCGTC189450.043.435769
CCTGGCC513900.043.35895
TGTTACG872100.042.3347433
CCGGCAT116100.040.439161
TGCCGCA120350.038.8316041
CAATCAA688200.038.696294
AGACGTG208400.037.8627784
CCTTGTT240850.037.3723261
Produced by FastQC (version 0.11.2)