FastQCFastQC Report
Fri 19 May 2017
H5JVVBGX2_n01_1c-brugia_2017-05-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JVVBGX2_n01_1c-brugia_2017-05-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133265731
Sequences flagged as poor quality0
Sequence length150
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT5286740.39670663720743027No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA3810150.2859062094515506No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG3009670.22583975470783257No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC2909130.21829542960297874No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT2522320.1892699631835584No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC2444260.18341249334384396No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG2409240.18078466098685192No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2308060.1731923115328126No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA2283940.17138239387288542No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA2174320.16315672331396286No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA2000950.1501473773478945No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC1989690.14930244895441275No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1846450.13855399930234127No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA1813760.13610100559160254No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA1744710.13091962854276468No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT1730030.12981807003332313No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC1575320.11820893399819342No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG1475850.11074489960213403No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC1438680.10795573544709705No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG1406270.10552375238912695No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA1405620.10547497765948546No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1393490.10456476616632973No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA1385950.1039989793024885No Hit
CTACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTGACGTGACG1351440.10140941634875361No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT1342040.10070405872009212No Hit
CCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTC1340090.10055773453116765No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1335410.10020655647774897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA413850.078.734551
TTACGAC485500.067.322755
GTTACGA501600.065.148374
GTGCGTC556850.064.230079
CTCACCC1311150.061.518772
CTCAGAC575950.060.800791
GTCCAAT107450.060.5831341
CAGGCGT620650.056.316427
TGTTACG580200.056.163333
GACAGGC624250.056.0150575
CCTCACC1474300.054.953451
CCAGACG359300.052.5176166
CAGACAG680000.050.5674973
CCTGGCC554450.050.1470155
AGACGTG363850.049.82354
CCTCAAC180000.049.566531
ACGACTT677200.048.2542767
ACGTGGC376550.047.9321446
TTGTGTC118300.047.654372
TGTGTCG118350.047.4506763
Produced by FastQC (version 0.11.2)