FastQCFastQC Report
Thu 23 Feb 2023
H5JMGDSX5_n02_f3HRB0245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JMGDSX5_n02_f3HRB0245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76465920
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10200891.3340439767153787No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2441530.3192964918227624No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1739770.22752227397512512No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCT1644550.21506966763755672Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1629420.2130910083864812No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1564320.20457741174107366No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)