FastQCFastQC Report
Thu 23 Feb 2023
H5JMGDSX5_n02_f3HRB0014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JMGDSX5_n02_f3HRB0014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440557389
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8885890.2016965376558467No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTAGGTGTGTAGATCT5481030.1244112603000741Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)