FastQCFastQC Report
Thu 23 Feb 2023
H5JMGDSX5_n01_f3HRB0245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JMGDSX5_n01_f3HRB0245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76465920
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTC5972340.7810459875458243TruSeq Adapter, Index 23 (96% over 32bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA2545500.33289339878471347No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1665950.2178683000217613No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1638490.2142771577194128No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG1634240.21372135455899832TruSeq Adapter, Index 23 (97% over 40bp)
GGGGAGCACACGTCTGAACTCCAGTCACCAACTCTATCGCGTATGCCGTC1552230.20299631522121228TruSeq Adapter, Index 23 (96% over 32bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT1380010.1804738633890758No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTAT1284170.16794017517869397TruSeq Adapter, Index 23 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)