FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35631520
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATC21500.09.31249638
CTCGTTT63250.08.9063411
AAACGAT23900.08.74559837
CCCGTAT23200.08.726091
CTCGTAT39350.07.88486431
TCGTTTT68500.07.83678772
CTCCGAT59000.07.79496431
CGTTTTG51950.07.75003623
CCCGTTT48400.07.7290021
GAAACGA29250.07.672499736
TGAAACG31100.07.640433335
CTATACG9450.07.450587342
CGTGAAA30300.07.406506533
CCCGATT23350.07.35068131
CCCAATT73100.07.2848041
CTCGATT27500.07.20161151
CGTATCT29800.07.16132973
ACGTGAA33600.07.1372432
CCCGGAT75650.07.1265121
CTAGCGA34650.07.111325728
Produced by FastQC (version 0.11.2)