FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26558374
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG748560.2818546045025196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG19050.023.7916132
CGTCCGC30500.014.86042533
GTCACGT34800.013.84546429
ACACGTC36400.012.87230313
ATCGGAA36750.012.57165052
GATCGGA36600.012.5059431
CTCGTAT34750.012.47481644
ACGTCTG38000.012.44587715
CGCACAT39050.011.88858637
CGTCTGA40550.011.88018216
GTCCGCA38250.011.79201234
AGTCACG40550.011.71942628
TCACGTC47250.010.38348630
CACGTCT47400.010.302714
CACACGT49700.09.91460312
CGCCTAT7600.08.39689351
CTCCGAT47000.08.1467721
CTCGTTT50000.08.0980791
CGGCTAT15050.08.0419321
GCACACG62850.08.01514611
Produced by FastQC (version 0.11.2)