FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33310650
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG892830.26803139536454557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT10500.010.2694941
ACCCGTC27400.010.11740132
CGATCTA10300.010.0390064
CTCGTTT62650.09.87023451
TCGTTTT67750.08.7352832
CTCCGAT53800.08.5079171
CGTCCCG33200.08.35004135
CCCGGAT68650.08.2061921
CGTTTTG49700.08.1449833
TTACGGC17050.08.12981840
CTCGTAT39100.07.87940651
CCCGTAT22100.07.8664191
CTCCTAT48550.07.7961721
TCGTATC25150.07.78549152
ATTACGG19350.07.73190939
CGCGTTT8850.07.7083361
CCCGAAT45750.07.4556051
ACACGTC40500.07.44115313
CTCGATT24650.07.4097471
CTCCAAT140900.07.38741351
Produced by FastQC (version 0.11.2)