FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37040429
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT69650.09.73172951
ACACGTC45950.08.90436813
GATCGGA43800.08.6922541
TCGTTTT78000.08.6593352
CTCCGAT60150.08.5247311
ACGTCTG50500.08.01487615
CTCGTAT45600.08.01045744
CGTTTTG63100.07.9843773
CGCCTAT12450.07.954331
CCCGTTT50250.07.92689131
TCGTATC28050.07.9218982
AATCTCG44600.07.89331341
ATCGGAA49800.07.7312462
CGTCTGA53450.07.654831416
CGTATCT33500.07.61812543
CCCGAAT47100.07.3823831
CTCCAAT154800.07.26457741
CCCGTAT23450.07.2261721
CCCGGAT80650.07.09462361
ACGCGAA12750.07.073601218
Produced by FastQC (version 0.11.2)