FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34934365
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1032610.2955857362800211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT70650.09.3128591
CTCGTAT42350.09.196951
CGTTTTG57650.08.891653
CGTACGA12550.08.764324
TCGTTTT76550.08.736862
CTCCGAT57800.08.7182971
TCGTATC28750.08.4939982
CCCGTAT26800.08.4571971
CCCGTTT48350.08.2377021
CTCGAAT67400.07.8356551
CCCGGAT87950.07.78123571
CCCGAAT47000.07.67838671
CTATACG14800.07.58204642
CGTTTAT29400.07.4082163
TATCTCG31250.07.392994441
TCCGTAT28650.07.372381737
CGCCTAT9300.07.3350451
GCGTACG16350.07.265170623
CTCGGAT80700.07.19870471
CGGCTAT20600.07.1570091
Produced by FastQC (version 0.11.2)