FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40613592
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1115510.27466420601260777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA52450.013.3806012
GATCGGA51700.013.1946151
ACACGTC56250.012.67069613
AATCTCG52850.012.40657641
ACGTCTG60500.012.28953915
CGTCTGA64550.011.348062516
CTCGTAT60350.010.75636544
CACACGT76550.09.71310512
CACGTCT73900.09.61470814
CGTACGA13350.09.22790124
TCTCGTA72000.09.16844443
CTCGTTT83700.08.8073441
TCGTTTT86450.08.7543492
CGTTTTG66500.08.5684793
CTCCGAT67200.08.3829391
AGTCAAC88750.08.28000334
CCCGTAT26650.08.1745451
GCACACG92200.08.04051611
CCCGAAT57250.07.7642851
AACCGGT46800.07.70864710
Produced by FastQC (version 0.11.2)