FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35425547
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT77700.012.1498591
TCGTTTT81100.011.2037882
CGTTTTG67300.010.3954683
CCCGTAT20400.09.6005221
TCGACTA17950.09.31405512
CTCGAAT80550.09.1246441
CCCGAAT42050.09.0534711
CGCATAT10250.09.0169651
CTCCGAT63850.08.9952781
CTCGTAT39600.08.9467571
CTCAAAT151600.08.5351891
CTCCAAT152350.08.2042881
CTCCTAT56450.08.1863511
CGATCTA10700.08.0188514
CTCGATT28000.07.93776751
CGACTAG20800.07.82619613
CTGGAAT171250.07.82567261
CTCAGAT121450.07.80934761
CCCAAAT96550.07.74928571
CCCGTTT46450.07.7221261
Produced by FastQC (version 0.11.2)