FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31083515
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA33650.010.19947052
GATCGGA33250.010.1262421
CGTATGC35500.09.79394444
ACACGTC37800.09.54388413
CTCGTTT58050.09.5152451
ACGTCTG42950.08.70659515
CTCCGAT49800.08.5285751
CGTTTTG49650.08.5078813
CTCGTAT38050.08.44267942
CGTCTGA42650.08.40678216
CCCGTAT20100.07.99236541
TCGTTTT65050.07.88037162
TAACGCG13700.07.867500316
CTCAAAT117850.07.78672741
ACGCGAA13600.07.763594618
TCGTATG50700.07.59533943
CGTACGA7601.8189894E-117.525795524
AACGCGA15750.07.40211517
CCCGTTT40700.07.3534821
CGCTAGA8101.0913936E-117.3354581
Produced by FastQC (version 0.11.2)