FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32320175
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1091200.33762193428717513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG177050.013.2620681
CTCGTTT68200.011.8419841
TCGTATG32850.011.05236143
ACGCCAA33100.010.9008732
CTCCGAT55150.010.4942891
TCGTTTT73150.010.1356952
GTCACGC36250.010.135529529
TCGACTA16400.09.9259512
GATCGGA34850.09.9137891
TATCTCG36050.09.76514939
CGCCAAT36750.09.69867533
CGTTTTG58100.09.4288443
CTCAAAT137200.09.415141
CGTATGC36700.09.29367744
ATCGGAA38900.09.1622762
ACACGTC40450.09.1362713
CCCGAAT39050.08.8475171
CTCGTAT40900.08.82395651
CGGCTAT18700.08.4729321
CGACTAG19750.08.46495413
Produced by FastQC (version 0.11.2)