FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30957401
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG805620.2602350242515514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT66950.010.9456881
TCGACTA17850.010.10566912
TCGTTTT74750.010.0953192
CGTTTTG57600.09.5870163
CGACTAG20550.09.20600413
ATTCGAC21800.08.67810910
CCCGTTT40550.08.3032871
CCCGAAT37450.08.2854611
CGCGTTT8800.08.2524081
TCGTATG26250.08.04723143
CGCGAAA2500.00578154257.92231131
CTCGTAT36150.07.91378931
CGCCTAT9500.07.87598281
CGCGTAG10150.07.80523251
CTCCGAT54200.07.7550311
CTCGAAT62650.07.72771361
CTCCAAT130850.07.70266771
CCCGTAT21200.07.6814871
CCCAAAT85500.07.67007451
CTCCTAT52200.07.419791
Produced by FastQC (version 0.11.2)