FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34220931
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1017200.297244981441329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42200.08.8646261
TCGTATC30400.08.5396552
TCGTTTT59450.08.4375212
CTCGAAT57150.08.3168991
CCCGTAT26500.08.3038221
CGTATCT35450.08.1298373
CCCGTTT46650.08.1133591
CTCGTTT57500.08.1131961
CGTACGA11100.07.92738924
CCCGAAT47150.07.79396871
CGTTTAT22100.07.66523083
CTCGATT28450.07.57997371
CTCCGAT49250.07.4616371
CCGAAAC24100.07.3959141
CCCGGAT87500.07.36857461
CTGGAAT168750.07.28940251
CCCAAAT98000.07.2302571
CTCAGAT107600.07.19870421
CCCGATT21050.07.10854531
CGCTAGT8057.2759576E-117.10724641
Produced by FastQC (version 0.11.2)