FastQCFastQC Report
Wed 19 Apr 2017
H5JK3BGX2_n01_da01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5JK3BGX2_n01_da01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36878429
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39000.017.8290843
CGTATGC42100.016.46440944
TATCTCG45550.014.82913739
GATCGGA48950.014.1158691
ATCGGAA50250.013.9665542
TCACCGA50800.013.68536130
ACACGTC55600.012.89802713
ACGTCTG57600.012.33545615
CGTCTGA57700.012.16154816
CTCGTAT56650.011.96237142
CGATGTA60800.011.651937534
GTCACCG62750.011.50001229
GATGTAT62650.011.34319935
ATGTATC59000.011.29921436
CCGATGT71000.010.00899833
CACGTCT71450.09.94439614
CACCGAT78400.09.40075631
TCTCGTA75150.09.2811841
CACACGT79650.09.22457712
ACCGATG79800.09.18076732
Produced by FastQC (version 0.11.2)