FastQCFastQC Report
Thu 27 Apr 2017
H5J5LBGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5J5LBGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126950205
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12894651.0157250238390714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA7182250.068.709254
GTGTCCA8140250.068.56264
GGGTCCA6028350.068.4806064
AGTCCAC29725450.068.39835
CGTCCAC27940750.068.3799445
CCGGTCC2272500.068.296533
TCCACGA111279600.068.147657
CCACGAG107124550.068.100188
CCGTCCA11030500.068.094734
GTGGTCC3582850.068.008183
GTCCACG111823800.067.9744956
ACGAGGT105797400.067.9736810
GCGGTCC2492850.067.8619463
ACGTCCA6030000.067.851394
CACGAGG106533100.067.738799
GGTCCAC29212700.067.683735
GTCTCTA23340750.067.66888415
CAGTCCA13340800.067.632794
TGGTCCA7855200.067.593614
TTCTTGT1307400.067.431461
Produced by FastQC (version 0.11.2)