FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_9w-54_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_9w-54_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15332177
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328200.21405962114838614No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG196530.12818140568035447No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC183640.11977424993202204No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA173210.11297156300765376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG33700.037.00526445
CTCGTAT32650.035.54917544
CGTATGC37550.034.2227546
GTCACGT39800.032.42867729
TATGCCG39100.032.15698648
ACGTCCG42800.030.171332
TCACGTC44150.029.31650430
GTCCGCA46650.028.05804334
CCGCACA48400.027.0445236
CGCACAT47000.026.74899137
GTATGCC48450.026.52282147
GCCAGTA71550.026.454321
AGTCACG48850.026.34785528
ACGTCTG50800.025.9869715
TCCGCAC57100.023.46765335
CGTCTGA58100.022.89995616
CACGTCT58600.022.76345414
ATGCCGT56200.022.37139749
CACACGT60250.022.19726812
TAGTCAT84750.021.8799426
Produced by FastQC (version 0.11.2)