FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_9w-30_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_9w-30_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18255792
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405520.22213224164692497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA222200.12171479604938532TruSeq Adapter, Index 22 (97% over 40bp)
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG197100.10796573492949525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC42350.042.98251338
TCGTATG51050.040.8070245
ACCGTAC53600.039.1018832
CTCGTAT48150.038.0986144
TACGTAA54850.037.97084436
CGTATGC55800.037.88937446
ACGTAAT53900.037.6800337
TATGCCG55700.037.14835748
CCGTACG58950.035.6180233
CACCGTA64450.032.836231
ACGTCTG69200.031.43070415
GTATGCC69600.030.37582447
ATGCCGT73050.028.74939349
CACGTCT77400.028.05645414
CACACGT79800.027.38520612
CAGTCAC79250.026.99325627
CGTCTGA81850.026.48890316
ACACGTC84050.026.28761113
CCGTCTT82200.025.91344352
TAATCTC73200.025.15232540
Produced by FastQC (version 0.11.2)