FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_9w-15_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_9w-15_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14039574
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289410.20613873326925733No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG179300.12771042768106783No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC166400.11852211470234068No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG161030.11469721232282404TruSeq Adapter, Index 11 (100% over 63bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA160940.11463310781367013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT34700.040.4161942
TATGCCG38950.037.9587846
TCGTATG39200.037.36499843
CGTATGC44350.033.02610844
ACGTCTG49000.031.23355115
CACGGCT48650.030.93892931
AGTCACG49200.030.57703228
ATGCCGT51050.029.50229547
GTATGCC51550.029.01687445
CACGTCT56750.026.9078214
GCCAGTA66400.026.896941
CACACGT57050.026.82664912
ACACGTC57700.026.82314113
CAGTCAC57400.026.80847727
CCGTCTT56350.026.17088350
CGTCTGA62000.024.74035316
GCTACAT58600.024.16444835
CCAGTCA65950.023.53598226
TCTCGTA58050.023.44438641
GCCGTCT65600.023.06222549
Produced by FastQC (version 0.11.2)