FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_9w-12_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_9w-12_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20845054
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG473670.22723376010443533No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG261980.12567969360981265No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC219160.10513765039898673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36200.047.52353742
GTCACGT40150.045.03882629
TCGTATG41550.043.400443
ACGTCTG47650.041.81137515
TATGCCG44850.040.6682346
AGTCACG48050.038.06052828
CACGTCT52650.037.97216814
ACACGTC53950.037.31265313
ACGTAGA49550.036.65267632
CGTATGC51700.035.14692744
CACGTAG51900.034.6556831
CGTAGAG54300.033.1334933
ATGCCGT56250.032.79437647
CAGTCAC58700.031.91697727
GTATGCC58500.031.18017845
GTCTGAA65750.030.82616217
TCACGTA61350.029.70535730
CTATAAG90300.027.39107546
GCCGTCT67000.026.6534649
TATAAGC92850.026.5270147
Produced by FastQC (version 0.11.2)