FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4w-4_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4w-4_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13957708
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294720.21115214618331318No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG218310.1564082011172608No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA180470.12929773283693854No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC159390.11419496668077596No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA153940.11029031414040184TruSeq Adapter, Index 15 (97% over 40bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG147860.10593429809536063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35300.040.41948745
CTCGTAT32950.037.74499544
CGTATGC39400.037.17631546
TATGCCG40250.034.84443748
ACGTCTG47050.031.35436615
GTATGCC48100.030.3796447
GCCAGTA74850.028.282111
GTCACAT52000.027.74041729
CACGTCT53950.027.72769214
ATGCCGT51900.027.02105949
CAGTCAC54950.026.8114827
AGTCACA57250.025.55591628
CCGTCTT56700.025.20924852
ACACGTC59700.025.05674213
TGTCAGA59050.024.91629835
CACACGT60500.024.66826412
GTCAGAA58250.024.42875336
CGTCTGA60550.024.19302716
CCAGTCA63750.023.48383126
AGTAGTC91350.023.1288974
Produced by FastQC (version 0.11.2)