FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4w-3_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4w-3_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16448246
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344230.20928067345296272No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG172740.10502031645197915TruSeq Adapter, Index 10 (100% over 63bp)
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG169730.1031903340939818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39200.040.9800743
CTCGTAT38550.038.62247542
TATGCCG44300.036.1058646
CGTATGC46900.034.3257644
ACGTCTG53300.030.84760715
TCACTAG54000.030.10510330
AGTCACT54700.029.20802528
GTATGCC56100.028.94352145
TATCTCG51350.028.72194139
CACGTCT59750.027.9216914
CAGTCAC61350.026.48910527
GCTTATC56400.026.45399936
CACTAGC63300.026.1236231
ATGCCGT62900.025.86775847
CTTATCT58600.025.2851137
CGTCTGA68550.024.23664716
TCTCGTA62950.023.81588441
ACACGTC71000.023.4974813
CCAGTCA69800.023.4247126
CCGTCTT68850.023.07515150
Produced by FastQC (version 0.11.2)