FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4w-30_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4w-30_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13199110
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1555961.17883705795315TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277570.2102944819764363No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG132460.100355251225272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG220400.063.19893345
CTCGTAT199150.063.0892844
CGTATGC225350.062.6062146
TATGCCG224200.062.47718448
ACGTCTG239850.060.63204215
GTATGCC234200.060.1063147
CACACGT246700.059.1432112
CACGTCT246900.058.9842914
ACACGTC247350.058.93227413
ATGCCGT238300.058.6604949
CAGTCAC246150.058.41089227
CACAGTC244650.058.30857531
AGTCACA246850.058.20796228
CCGTCTT239500.057.9361452
ACAGTCA247350.057.80538632
GTCACAG249100.057.534429
CGTCTGA253300.057.3855316
CCAGTCA251850.057.07332226
TCTCGTA219200.056.89304743
GTCTGAA259050.055.9261217
Produced by FastQC (version 0.11.2)