FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4w-18_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4w-18_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12732726
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268840.21114096070236646No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA158100.12416822603423651TruSeq Adapter, Index 14 (97% over 44bp)
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG128420.10085821370851772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT29500.042.85479439
CTCGTAT33700.038.44130344
CGTATGC37250.037.5607346
TCGTATG36400.037.20446845
TTCCGTA36950.036.82955636
TCCGTAT37350.035.41915537
GTTCCGT38900.035.07171635
TATGCCG39200.034.8966448
TATCTCG36050.034.78411541
CCGTATC38000.033.35922638
AGTTCCG42500.032.18131634
ACGTCTG46200.031.55939715
GTATGCC46250.030.02693747
GTATCTC43600.029.39329340
ACACGTC55050.026.98672913
CACACGT53600.026.94497912
CACGTCT55400.026.94077714
AGTCACA53500.026.89574428
CAGTCAC54450.026.2347227
ATGCCGT52500.026.12061949
Produced by FastQC (version 0.11.2)