FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4w-13_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4w-13_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15794922
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG319760.20244481106016224No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG196720.12454635736726019No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG179950.11392902098535214TruSeq Adapter, Index 9 (100% over 63bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA167850.106268331049688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC43000.039.76588444
CTCGTAT43300.035.5003242
TCGTATG48700.035.11167543
ACGTCTG52050.033.9069415
AGTCACG51850.033.20965628
TATGCCG52800.032.45062646
CACGTCT58450.030.60710314
GTCACGA57000.030.21152729
GCCAGTA68750.028.1342541
GTATGCC63750.027.2026545
CGTCTGA70150.025.30589316
CAGTCAC68500.025.28625127
ACACGTC70750.025.2858513
ATGCCGT69650.025.1451347
CACGATC69150.025.06055631
CACACGT73100.024.37842612
GCCGTCT72700.023.80277649
ATCGGAA72800.023.6741242
TCGGAAG75200.023.1014043
CCGTCTT76100.022.55568950
Produced by FastQC (version 0.11.2)