FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-8_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-8_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14709602
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1422050.9667494742549798TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334360.22730730579930036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT192400.063.3783542
TCGTATG199900.063.05750743
TATGCCG200400.062.7097146
TATCTCG195050.062.10507639
AGTCACG209750.061.691828
GTCACGC210350.061.2413329
CGTATGC210200.060.0828744
CGCCAAT212250.060.02002333
ACGTCTG228000.059.98872815
GTATGCC211500.059.73132745
GCCAATA212600.059.3760234
CAGTCAC219700.059.33316427
TCACGCC218150.058.85640730
ATGCCGT213700.058.8537547
CACGCCA218350.058.6091731
CACGTCT237600.057.62297414
ACACGTC238050.057.51384713
ACGCCAA223050.057.25797332
ATATCTC211550.057.2434538
CCAATAT219800.057.1497635
Produced by FastQC (version 0.11.2)