FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-6_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-6_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15511192
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1080520.6966066824522577TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG393000.25336544090228524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG167250.062.99880243
CTCGTAT145300.062.6227142
TATGCCG169600.062.26766246
ACGTCTG230450.061.0862315
CACGTCT233650.060.2649514
GTATGCC175950.060.08108545
GTCACAT185150.059.97362529
AGTCACA190150.059.78948228
ACACGTC237850.059.22957213
CAGTCAC198050.059.0730327
ATGCCGT177850.059.0280747
CGTATGC180500.058.92945544
GTCTGAA244950.057.14800617
CCGTCTT187550.054.72942450
GCCGTCT189600.054.59853749
CACGATC156500.054.46336736
AGCACAC259050.054.4618610
CACACGT260350.054.1106512
CTCCAGT256050.053.91305524
TCCAGTC254900.053.8496425
Produced by FastQC (version 0.11.2)