FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-5_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-5_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14144966
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG970650.6862158594089233TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368680.26064396337184553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT135050.061.92752542
TCGTATG144350.061.45585643
TATGCCG145900.060.87443546
ACGTCTG187300.060.23296715
ACACGTC194250.058.0783313
CACGTCT194850.058.00562714
CGTATGC154350.057.8549544
TATCTCG141450.057.60549539
GTATGCC155600.057.2363945
ATGCCGT159200.055.5272447
GTCTGAA203550.055.50984617
CAGTCAC174600.055.25232327
CACCGAT163850.055.24420531
TGTATCT151150.054.08881837
CACACGT208900.054.02145812
CGATGTA164300.053.9804334
AGTCACC176950.053.46977628
TCCAGTC211950.052.36279725
CCAGTCA194400.052.07854526
GTATCTC157400.051.83245538
Produced by FastQC (version 0.11.2)