FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-4_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-4_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17053355
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG349960.20521475099767758No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG225990.1325193781516892No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA194720.11418281036194931No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG193140.11325630645699922TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC48700.037.9495144
CTCGTAT44750.036.82085842
TCGTATG51100.036.2350743
ACGTCTG55000.034.59650815
TATGCCG53650.034.38311446
CACGTCT64600.029.9888414
GTATGCC64750.028.75654645
GCCAGTA80300.026.6218191
ATGCCGT70650.026.30471447
CGTCTGA73600.026.1344616
CAGTCAC72050.026.09913327
ACACGTC75600.025.7165813
TGTAATC64100.025.0529536
CTTGTAA74800.024.83901834
TCGGAAG76750.024.7018073
CACACGT80200.024.1984412
CCGTCTT76550.024.09185450
TAATCTC67500.023.79284138
CCAGTCA80900.023.7069926
ATCGGAA80450.023.5660172
Produced by FastQC (version 0.11.2)