FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-3_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-3_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685085
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265480.19399221853572704No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG139370.10184079967351317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28650.031.47043246
GTCACGT31450.028.64029929
AGTCACG35150.025.3281128
CTCGTAT30800.024.90041544
GCCAGTA55800.024.8405081
ACGTCTG37350.024.45521515
TCGTATG36750.023.78270145
TATGCCG38600.023.08848448
CGTGAAA40800.022.93861633
CACGTCT44600.020.94364514
TCACGTG43950.020.73388130
CACGTGA44850.020.09112231
TGAAACG49500.018.28067835
GTATGCC50200.018.16676147
AGTCATA74200.017.9771867
CAGTAGT74950.017.8893363
AGTAGTC75550.017.7017064
GAAACGA48200.017.62717436
CGTCTGA53050.017.3477116
TAGTCAT77600.017.145116
Produced by FastQC (version 0.11.2)