FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-33_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-33_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14704218
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297930.20261533119272307No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG174070.11838099788781696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35300.035.13207242
CGTATGC39750.034.9372944
TCGTATG40100.034.3740243
TATGCCG42300.032.99212346
ACGTCTG47750.031.04016315
GTATGCC48900.028.61188545
ACACGTC53950.027.7925413
CACGTCT55750.026.83337214
CACACGT55100.026.77439312
GCCAGTA69800.025.0938451
ATGCCGT60500.023.46665847
CGTCTGA63550.023.26846516
CCGTCTT61500.022.79958350
CAGTCAC64450.022.59282927
TCGGAAG65800.022.3675863
ATCGGAA69200.021.2192252
CCAGTCA69950.020.8104626
TCTCGTA55600.020.68839641
GTCTGAA72850.020.67682317
TAGTCAT83250.020.535946
Produced by FastQC (version 0.11.2)