FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13116221
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1245100.9492825715577681TruSeq Adapter, Index 8 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278880.21262221793914576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG188400.063.7166143
CTCGTAT167850.063.61332342
TATGCCG191500.062.774446
CGTATGC196550.061.54911844
GTCACAC204200.061.47987429
ACGTCTG237400.061.43215
ACACGTC242250.060.44398513
GTATGCC199250.060.43837445
AGTCACA210550.060.34292628
ATGCCGT199250.060.12262347
CAGTCAC215500.059.92880227
CACGTCT245700.059.56719614
TCACACT210500.058.9796530
CACACTT209000.058.64221231
GTCTGAA250450.058.02655817
CCGTCTT207800.056.65770350
GCCGTCT208750.056.6358149
CACACGT258900.056.5033312
CCAGTCA239600.055.5266626
TTGAATC189900.055.0736
Produced by FastQC (version 0.11.2)