FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-19_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-19_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10945247
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1651301.5086913981932066TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245250.2240698633845358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT218600.065.0106742
ACTTAGG246150.064.8878932
TCGTATG240350.064.7918343
TATGCCG243400.064.1765846
ACGTCTG276200.063.34859515
AGTCACT257800.063.12254728
TTAGGCA247350.063.06601734
GTCACTT256400.062.89312429
GTATGCC249850.062.5912545
ACACGTC283200.061.96535513
CAGTCAC265550.061.8726927
CACGTCT283450.061.83801314
ATGCCGT252350.061.68058847
CGTATGC254950.061.65025744
CCGTCTT256150.060.17355750
GTCTGAA291050.060.0585717
CACACGT294050.059.76098312
CTTAGGC266400.059.665933
TCTCGTA230100.059.3270841
GCCGTCT262900.058.71305549
Produced by FastQC (version 0.11.2)