FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-17_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-17_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14952194
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1273250.8515472712566464TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354320.23696856795731783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG188950.064.5636343
CTCGTAT169200.064.09321642
TATGCCG193100.063.6048346
GTCACAC207350.061.52910629
CACAGTG204350.061.3848733
ACGTCTG237950.061.03843315
AGTCACA211600.061.0379728
GTATGCC203200.060.7672345
ATGCCGT202100.060.61606647
ACACAGT207000.060.5846132
CAGTCAC217150.060.44219627
CACACAG213600.059.3656531
CACGTCT245550.059.1073314
ACACGTC248900.058.50565713
GTCTGAA250700.057.68844217
CGTATGC217150.057.1019344
GCCGTCT213350.057.04216449
CCGTCTT214300.056.55812550
ACAGTGA220750.055.18555534
CTCCAGT261500.055.0981224
Produced by FastQC (version 0.11.2)