FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-14_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-14_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12061701
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1365741.132294690442086TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251340.20837856949032313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT189450.064.5695942
TCGTATG203400.064.2863643
TATGCCG204800.064.2672846
GTATGCC211000.062.33258445
ATGCCGT212450.061.67437447
CGTATGC215000.061.31644
ACGTCTG243900.061.2769715
CAGTCAC226650.061.01425627
CACGTCT251350.059.48814814
ACACGTC251850.059.3563613
AGTCACC231000.059.30237228
GCCGTCT219000.059.11504749
GTCTGAA253050.058.9542517
CCGTCTT221050.058.37470250
CATCTCG206100.057.6845139
CCAGTCA245650.057.09549326
TCTCGTA210750.056.66418541
TGCCGTC232000.056.5807748
ACCAGAT237300.056.41077432
CTCCAGT262350.056.389224
Produced by FastQC (version 0.11.2)