FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-11_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-11_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17104546
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403490.23589635176519738No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG227750.13315173638633848No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC224150.131047032759595No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA210030.1227919174235902TruSeq Adapter, Index 20 (97% over 44bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA209280.12235343750135198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG47750.044.20816445
CTCGTAT46000.042.9604744
GTCACGT51350.041.73336429
TATGCCG51250.040.8479448
CGTATGC53650.039.92431646
GCCTTAT53350.038.26371837
ACGTCTG59450.036.64103715
TCACGTG58800.036.6296130
TATCTCG52550.036.28819341
CACGTGG61200.035.08816531
AGTCACG62800.034.34192728
CCTTATC57950.033.1408138
GTATGCC65000.032.8991947
CACGTCT67800.032.5860814
CAGTCAC69850.030.92183127
ATGCCGT68450.030.17857649
ACACGTC74500.029.93310713
CACACGT75250.029.49718712
CTTATCT66150.028.87718239
CGTCTGA77800.028.35333316