Basic Statistics
Measure | Value |
---|---|
Filename | H5HVHBGX2_n01_4s-11_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17104546 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 40349 | 0.23589635176519738 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 22775 | 0.13315173638633848 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC | 22415 | 0.131047032759595 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 21003 | 0.1227919174235902 | TruSeq Adapter, Index 20 (97% over 44bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA | 20928 | 0.12235343750135198 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTATG | 4775 | 0.0 | 44.208164 | 45 |
CTCGTAT | 4600 | 0.0 | 42.96047 | 44 |
GTCACGT | 5135 | 0.0 | 41.733364 | 29 |
TATGCCG | 5125 | 0.0 | 40.84794 | 48 |
CGTATGC | 5365 | 0.0 | 39.924316 | 46 |
GCCTTAT | 5335 | 0.0 | 38.263718 | 37 |
ACGTCTG | 5945 | 0.0 | 36.641037 | 15 |
TCACGTG | 5880 | 0.0 | 36.62961 | 30 |
TATCTCG | 5255 | 0.0 | 36.288193 | 41 |
CACGTGG | 6120 | 0.0 | 35.088165 | 31 |
AGTCACG | 6280 | 0.0 | 34.341927 | 28 |
CCTTATC | 5795 | 0.0 | 33.14081 | 38 |
GTATGCC | 6500 | 0.0 | 32.89919 | 47 |
CACGTCT | 6780 | 0.0 | 32.58608 | 14 |
CAGTCAC | 6985 | 0.0 | 30.921831 | 27 |
ATGCCGT | 6845 | 0.0 | 30.178576 | 49 |
ACACGTC | 7450 | 0.0 | 29.933107 | 13 |
CACACGT | 7525 | 0.0 | 29.497187 | 12 |
CTTATCT | 6615 | 0.0 | 28.877182 | 39 |
CGTCTGA | 7780 | 0.0 | 28.353333 | 16 |