FastQCFastQC Report
Wed 19 Apr 2017
H5HVHBGX2_n01_4s-10_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HVHBGX2_n01_4s-10_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14817862
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1024960.6917057265076433TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354870.23948799091258913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG162200.063.59913343
CTCGTAT141500.062.69546542
TATGCCG164850.062.47134846
ACGTCTG209700.062.2295615
ACTGACC166350.061.7736932
AGTCACT178700.061.19241328
ACACGTC216300.060.4896813
CACGTCT216150.060.3726114
CAGTCAC188200.059.84090827
GTATGCC174400.059.26976845
ATGCCGT174100.059.03196347
GTCTGAA220400.059.02216317
CGTATGC179900.057.7066144
CTGACCA182850.056.2832733
CACACGT236900.055.22933612
GCCGTCT186100.054.4033849
CTCCAGT239300.054.25400524
TCTGAAC240550.054.24930618
AGCACAC241850.054.18354410
CCAGTCA217550.054.09817526
Produced by FastQC (version 0.11.2)