FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17815959
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10968226.1564016845795395No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1498790.8412626005706457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG26200.047.200669
GCAAAAG101300.040.1105353
CGCACTT12750.036.533175
GAGCAAA114150.036.1634181
CAAAAGC140700.028.8653644
ACGCACT17950.026.732714
AGCAAAA171800.024.429932
TTTAAAC56250.024.1194363
GCAGGTA49950.023.7842779
GCAGGTC46300.023.1083589
TAAACTA60350.022.5788365
AAAGCAG212800.021.3615886
TAGTAAC48150.019.9485262
AAGCAGG236250.019.2628787
AAAAGCA224600.018.9127275
CACGCAC27450.018.502723
CAGGTCA32350.018.2548588
GCACGCA26300.017.9962162
ACCTTGG40200.017.9738528
TCGACAC34350.017.824243
Produced by FastQC (version 0.11.5)