FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_Mirella6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_Mirella6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084360
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATTCGAATTCTTTTGGTCG39550.36473127005791434No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA37830.3488693791729684No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC29030.26771551883138445No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCA28190.25996901398059685No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGA23010.21219890073407355No Hit
CACTAAGACCACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGG22270.20537459884171308No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT22010.20297687114980267No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATTC20480.18886716588586816No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATG20190.18619277730642958No Hit
ATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCC18820.17355859677597846No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT18450.1701464458297982No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC17280.15935667121620126No Hit
GAGGAGGGAACAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG17120.15788114648271792No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG17090.15760448559518977No Hit
ATTCGACACTAATTGATGGCCATTCGAATTCTTTTGGTCGCTGTCTGGCT16500.15216348814046995No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG16430.15151794606957097No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC16140.14884355749013242No Hit
GGATTGGGTGATGCCCCATTCCTCGATCGGCTACGCCGAGATCAAAAGTC15830.14598472831900844No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATTCGAAT15820.14589250802316572No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA15440.1423881367811428No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14680.13537939429709692No Hit
CTATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCTTTTGCAAGA14610.13473385222619794No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT14500.13371942897192815No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA14230.131229480984175No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTGGACACAGTAC14210.13104504039248957No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAA13950.12864731270057916No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG13510.12458961968349995No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA13500.12449739938765723No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT13230.12200745139990408No Hit
CCATATGGCCATCATCAAAAAGTACACATCGGGAAGGCAAGAGAAGAACC13150.12126968903316243No Hit
CAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCA12500.11527536980338632No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC12480.1150909292117009No Hit
CACTATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCTTTTGCAA12370.11407650595743112No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT11670.10762108524844148No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAAATGAGGAATC11660.10752886495259877No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGT11550.10651444169832898No Hit
TTCTTAATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCCGGGCCCT11520.10623778081080085No Hit
ACTAAGGGCTTTCACTGAGGAGGGAACAATAGTTGGAGAAATTTCACCAT11240.10365561252720498No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG11150.1028256298646206No Hit
TTTTTGGACAATATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11140.10273340956877791No Hit
ATGTCCCCCTGCCCAATTAGCACATTAGCTTTCTCTCCTTTTGCAAGATT10960.10107344424360913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1100.0105.1425251
GCGAAAG4900.078.186342
GTAGAAA11000.065.056931
CAGGTAC3900.055.601653
TACCGTC400.00612796454.20166
GCAGGTA5950.052.2278759
TAGAAAC14350.049.8694272
TACTCCT3500.049.5580375
CAGGTCA6500.048.9204189
GTACTGA4500.048.17926
CAATTCG12800.047.9909979
ATGAGTC5750.047.760259
GCAGGGT7350.047.195959
TTTAAAC13700.046.429413
CCGCGCG803.7237147E-545.33542145
GGTACTG4800.045.1700865
AGGTACT5150.044.909034
GCTTTTA1801.8189894E-1244.327965145
CAGGGTG5700.043.1077048
AGGTCGT8050.043.0919536
Produced by FastQC (version 0.11.5)