FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_Mirella5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_Mirella5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316468
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG64390.4891117748399505No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC59220.44984002649513694No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT43410.32974595660509787No Hit
GAGCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAA42510.3229094820383025No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT41180.3128066918451493No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC36660.27847239735413243No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA35100.2666225081050204No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCT34600.26282446667902293No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT32500.24687269268983372No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG32170.24436598534867537No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC31260.23745354995336004No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGA29510.224160404962369No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA27640.20995573002913856No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC26900.20433462871866237No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG26690.2027394513197434No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT26450.20091639143526466No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCACAGTCCCGCACTCGCGAGATA26370.20030870480710508No Hit
GTCCCAATAGTTCTCATTGCATGCACCATCTGCCTAGTCTTATTAGCAAC25620.1946116426681089No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA24860.1888386197005928No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT22340.1696964909135657No Hit
CCCTTGGCCTCGACATCAAAACGGCCACTCTTGTTGGGAAACAAATTGTG22020.16726574440092734No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGT22000.16711382274388745No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT21750.1652148020308887No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT21590.16399942877456952No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTTTTCAACCTGTGT21540.16361962463196977No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT21160.16073311314821173No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC21100.16027734817709202No Hit
GTCTTATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGC20490.15564373763737516No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC19910.15123800958321812No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT19910.15123800958321812No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC19390.1472880465001808No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT19310.1466803598720212No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG19310.1466803598720212No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG19200.14584479075830176No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCATCTTTT18870.14333808341714346No Hit
CTTTTCAGACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG18050.13710929547850764No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC17830.13543815725106878No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCACA17620.13384297985214985No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT17450.1325516457673107No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT17230.13088050753987185No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA16570.1258670928575552No Hit
TATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCCA16570.1258670928575552No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC16530.12556324954347542No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT16350.12419595463011633No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC16200.1230565422023171No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT16160.12275269888823731No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC16130.12252481640267748No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA16120.12244885557415751No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGA15480.11758736254888079No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA15410.11705563674924116No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC15400.1169796759207212No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCATCAGGC15300.11622006763552173No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGTAGTAG15250.11584026349292197No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG15220.11561238100736212No Hit
CACTAAGACCACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGG15070.11447296857956289No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT15020.11409316443696314No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA15000.11394124277992325No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT14980.11378932112288336No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA14970.11371336029436341No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC14870.11295375200916391No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC14850.11280183035212402No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGT14840.11272586952360407No Hit
GTTCACAAGTGGCACAAACTAGACCGAAAGCAGCTTCTGTGGTCACTGTT14790.11234606538100432No Hit
GCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC14770.11219414372396443No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT14720.11181433958136469No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG14530.11037108383948566No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG14330.10885186726908666No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT14320.10877590644056673No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC14200.10786437649832734No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGCACCATCTG14140.10740861152720765No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGA14130.1073326506986877No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT13970.10611727744236851No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATG13940.10588939495680867No Hit
GTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTAGTG13670.10383845258677006No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13620.1034586484441703No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGTA13610.10338268761565035No Hit
TTTTCAACCTGTGTCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT13520.10269904015897083No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT13510.10262307933045088No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT13480.10239519684489103No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT13370.10155962773117158No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG13310.1011038627600519No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG13260.10072405861745214No Hit
CTTTCTATCATCCCATCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT13200.10026829364633245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2400.0126.566061
AGTAGAA7300.091.1473851
GAGCAAA35800.074.949741
CCGCACG809.822543E-1172.53594145
GTAGAAA12850.068.102251
CAAAAGC40000.066.5021744
GCAGGTA12800.064.938589
GCGAAAG8500.064.658312
GCCGTGC451.209171E-464.280192
AAAAGCA43550.061.742795
GCAGGGT22650.059.9935579
TAGAAAC14600.059.932472
AGCAGGG24150.059.8587728
AGAAACA15000.058.800723
CAATTCG8900.058.473289
AGCAAAA47150.057.9748732
AAGCAGG55150.053.9966357
AGCGAGC1750.053.883846145
CAAGGTA5650.052.4505929
CAGGTAC3300.050.38813
Produced by FastQC (version 0.11.5)