FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_Mirella4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_Mirella4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152206
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG48200.4183279726021215No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC45010.3906419511788691No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT38940.3379603994424608No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28610.24830629245117625No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG25950.22522014292583095No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23670.2054320147612493No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC23430.20334905390181965No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA23170.20109251297077083No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT23060.20013782257686558No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC22890.19866239196810292No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC22250.19310782967629053No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT21510.18668536702638244No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTTTTCAACCTGTGT18880.16385958760846583No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA18420.1598672459612257No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT17420.1511882423802688No Hit
CCCTTGGCCTCGACATCAAAACGGCCACTCTTGTTGGGAAACAAATTGTG17380.15084108223703052No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT17350.15058071212960183No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA17290.1500599719147444No Hit
GTCCCAATAGTTCTCATTGCATGCACCATCTGCCTAGTCTTATTAGCAAC17090.14832417119855304No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC16950.14710911069721908No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT16470.14294318897835978No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT16290.14138096833378752No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT15750.13669430640007083No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15260.13244159464540195No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC14930.1295775234636862No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGT14080.12220037041987283No Hit
GTCTTATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGC14080.12220037041987283No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT14070.12211358038406327No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT14030.12176642024082499No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCACAGTCCCGCACTCGCGAGATA13990.12141926009758672No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA13710.11898913909491879No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA13690.11881555902329966No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC13500.11716654834291786No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATGATAGGCCCTCTTTGCGTGCGA13180.11438926719701163No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC13180.11438926719701163No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG13000.1128270465524394No Hit
CTTTTCAGACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG12810.1111780358720576No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT12780.11091766576462889No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT12770.11083087572881932No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG12550.1089214949410088No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC12390.10753285436805571No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC12350.10718569422481744No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA12270.10649137393834089No Hit
TATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCCA12230.10614421379510261No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT12200.10588384368767391No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC12150.10544989350862606No Hit
CATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCA12140.1053631034728165No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA12100.10501594332957823No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT12050.10458199315053038No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGTCTGATG11960.10380088282824426No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT11950.1037140927924347No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCATCTTTT11870.10301977250595815No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC11870.10301977250595815No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGATACAGTAC11850.10284619243433901No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT11830.10267261236271986No Hit
CTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCG11750.10197829207624332No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT11590.1005896515032902No Hit
TTTTCAACCTGTGTCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT11580.10050286146748064No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT11580.10050286146748064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1000.0108.4687651
AGTAGAA5450.083.590611
GCGAAAG4400.077.2294852
GTACTGA2100.075.723056
CAGGTAC2200.072.29683
GTAGAAA9050.067.917831
GATCGTA350.00356139962.165916145
CATACTC4550.055.612923
GTCATAC4850.055.1662451
CTACACT4400.054.2131734
TACTCCT4800.054.213175
CAATTCG5400.053.5392239
AAGGTAG4350.053.1746065
AGGTACT3050.052.1394464
TAGAAAC12200.050.3729062
TCATACT5600.047.7696042
CCATGTC18150.044.601279
GTTTAAA8700.044.052451
ATCCAAC19050.043.653221
CAAGGTA5400.042.8351024
Produced by FastQC (version 0.11.5)