FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_Mirella3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_Mirella3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186869
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC27900.2350722784064627No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCACAGTCCCGCACTCGCGAGATA23050.19420845940032136No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT20610.17365016695187083No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19030.16033783003852994No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA17720.1493003861420258No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16830.14180166471615654No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC16750.1411276223407975No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC16370.1379259210578421No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT16340.13767315516708248No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA16210.13657783630712403No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAA15640.13177528438269093No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG15600.13143826319501142No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGATACAGTAC15350.12933188077201443No Hit
CACTAAGACCACTGTGGACCATATGGCCATCATCAAAAAGTACACATCGG15110.12730975364593733No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCACA14830.12495060533218073No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC14820.12486635003526084No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC14460.12183315934614519No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCTAAAATGAGGAATC14280.12031656400158736No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG13470.11349188495107716No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAGCTGA13180.11104848134040066No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT13060.11003741777736212No Hit
CTGTAGATACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT12990.10944763069892297No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG12860.10835231183896454No Hit
CCTTAACCCTGATCCTCTCACATTCACAGTCAATGAAGAAAATTGCATCC12780.1076782694636055No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA12550.10574039763444829No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG12300.1036340152114513No Hit
GTCCCAATAGTTCTCATTGCATGCACCATCTGCCTAGTCTTATTAGCAAC12070.10169614338229409No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC11880.10009529274081638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1850.097.706691
GCGAAAG4550.077.8581542
GTAGAAA7050.073.841321
CAGGTAC4000.066.866133
GTACTGA4350.061.4731336
AGTTCCG502.0046927E-458.033337145
AGGTACT4800.057.220534
CAATTCG5250.056.443749
AGCGAGC1051.1041266E-955.269848145
GCAGGGT7100.054.970119
CACGGGC400.00603741754.406254145
TTTAAAC5750.054.0586363
AGCAGGG8500.052.7163938
GGTACTG5150.051.9239045
CTACACT5050.051.5253144
GTGTTTT5600.049.0440949
AGGTCGT2850.048.1816446
GGTCGTT2900.047.3509257
CCTTATA3900.046.344142
AGTAGAA6450.044.8390431
Produced by FastQC (version 0.11.5)