FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_Mirella2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_Mirella2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050071
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG26520.2525543510867361No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTTGAAAAAAGAATGGAAA22830.21741387010973542No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC22440.2136998355349305No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGATACAGTAC17760.16913142063727118No Hit
GAGCAAAAGCAGGTAGATGTTTAAAGATGAGTCTTCTAACCGAGGTCGAA15880.15122786935359608No Hit
CTGTAGATACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT15700.14951369954983995No Hit
GTATCTTGGGTAGTCATAAGTCCCATTCTTGACACTTTCCATGCATGTGT15490.14751383477879115No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGTT15350.14618059159809194No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14680.13980007066188857No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCT13860.1319910748892218No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC12590.11989665460716466No Hit
GTTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC12170.11589692506506703No Hit
GTCTATGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAA12050.11475414519589629No Hit
GTTGTATATGAGGCCCATGCAACTTGCAAGTGCACCAGTTGAATAGCTTA11800.11237335380179055No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG11600.11046872068650596No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT11580.1102782573749775No Hit
GCTTTACTCCATCTATTTTTTCTCTGCTTAGTTTTGCTTCCTCTGAGTAT11260.10723084439052218No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11240.1070403810789937No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC11170.10637375948864411No Hit
GTCCTACATTGTGGAAACGTCTAATTCAGACAATGGAACATGTTACCCAG11140.1060880645213514No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTCGGTCTAGT10990.10465958968488796No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATCA10960.10437389471759528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2000.0101.214861
AGCAGGG14250.071.011038
GCAGGGT11750.067.6655359
GCAGGTA6350.064.880459
CAATACG1900.064.686181
TCTATGA2400.063.2562682
ATCCCGT350.003606521861.9682771
TGTAGCG350.00360720361.9653242
CTAACAC350.003607884561.9623683
GCGAAAG4000.061.4489482
ACGGATA2050.059.9416165
CGGATAG2100.058.5116466
CAATTCG1500.057.823279
CAAAAGC26400.057.5029564
TACGGAT2150.057.159094
GTAGAAA3950.056.736182
GCAGGGG4050.051.75549
ACGAGGG1152.5484042E-950.28834
GTGTTTT4300.048.7463659
GGATTAA450.00962454748.352024145
Produced by FastQC (version 0.11.5)