FastQCFastQC Report
Wed 8 Apr 2020
H5HNMAFX2_n02_Lib_prep_NC_water.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5HNMAFX2_n02_Lib_prep_NC_water.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAAATCACACTGAAGTTCGCTTTCAATATGATTTACTTATCCATGAT12.941176470588235No Hit
GTGTATGTTGATGGCGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG12.941176470588235No Hit
CTCTGAAGAACAAAGGGTCTAGTTTAAATAAGGAAAGTTCTGGCTTTTGC12.941176470588235No Hit
GGCTCAGCATTCCAATCGTCATAGGGAAGAATGCCTTCTAAAATGGGCTG12.941176470588235No Hit
AACATGCACCATTTACACAGGCACATTCAGCCTAGTGGGTCAGCCCTAGT12.941176470588235No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA12.941176470588235No Hit
GATCTATGGGAAATTCAGCTCTTGTCAAGAAATACCTAAAATGCTCGAAA12.941176470588235No Hit
CCTAAGTAACTAGCTCTCATAGTCAAGTAACTAGATCCTGGTGAGCATGC12.941176470588235No Hit
ACTTGGGAGGCTGAGGCAAAAGAATTGCTTGAACTGGGATGTAGGGGTTG12.941176470588235No Hit
GTCCTGCAGTTCTGCCAGTACTTGCTTCCATGACAGAAGATAATTTGGAT12.941176470588235No Hit
GAAAAAAAGTATTACAGAAATCACAAAAGAATTAAAATAATATTTAAAGG12.941176470588235No Hit
GTATTGAATGGAATCGTTGAGTTTACTCTAAGGGGATAATCATTGAATGG12.941176470588235No Hit
ATCGGGGGGTACGACTCGGACTGCGTGCCTGGCCGGCTGGCGGAGCGCCG12.941176470588235No Hit
GACAAAGATTCTGTCTTAATTTACTTCTAGACAAGAATAAAAACCATGTC12.941176470588235No Hit
GAAAAAATGTCCTTAATTAAAATTGAGAACAGTAAATAAAGTGATCAAGT12.941176470588235No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG12.941176470588235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.941176470588235No Hit
CTTTCGCACTGAGTAGCGGCTTTGGGTCCGGCATCATCACCTCAAACGCA12.941176470588235No Hit
CAATTAATACATAAGAACACAAACAATATCCAAAATACTCAATTATATGT12.941176470588235No Hit
GGTGTGGATGGCATGCCATTCTGCCGCATTTGAAGATCTAAGAGTATTAA12.941176470588235No Hit
GAGGGACGGGACGCAGTGGCTCACATCTGTAGTCCCAGCACTTTGGGAGG12.941176470588235No Hit
CATATTAGTCATTGCCTCCTCCATTCACTTCAACAAATTGAGAGTATAAT12.941176470588235No Hit
GTAAAACATTAAGCCTTTACAATGACACTGTAAGTCTCTCCGGTACTCTA12.941176470588235No Hit
GCCTCGTACAAAATTTTCAAGATCGAAAAGGGGAAGGTTACTAAATCAAT12.941176470588235No Hit
GTAACTAAACTGGAATCTTCCAGAACCAGGCACAAGTGCATGCAGGCAAT12.941176470588235No Hit
TCTTGTATCCCTTCATGTCAGAACAATAAAAGCTGGATCAAAAATAGATT12.941176470588235No Hit
GAGCAAAAGCAGAGCTTTAAAATTAATCCAAATCAGAAAAAAACAACCAT12.941176470588235No Hit
TCACTGGCCTATGGGTGAGGGGCCCGCGCGGGGGGAGATTGGTCCATAGG12.941176470588235No Hit
CACCTGCCACCATGCCCAGCTAATTTTTATATTTTTAGTATAGACGGGGT12.941176470588235No Hit
GATAAGAATTTGAATCACCAAATTCTCAGAATAATCAAAGATCATTTTAA12.941176470588235No Hit
CTTCTTTCTTTAACCAACAAGATAAGAAATCCATACGAGCATCCAGTCGG12.941176470588235No Hit
AGCAAATACAATGTATATGAAAACAACTACAATAGAGCCAAAAGAGATAA12.941176470588235No Hit
TTCTCATACAGATTCTTCACATTTGAGTCATGGAAATCCAGACTCCTTTC12.941176470588235No Hit
TTCGCGCTCTCTGTATGGTGGCTAGAGGGCCGTTGAGTGTTGCTTACCGC12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)